76 



Bulletin No. 207. 



CHAjRT I. 



Serological Relation of Botulism Antitoxin (Goat Serum H clear) 

 to an Organism c-91. 



Serum administered intraperitoneally March 15, 1917, 10:30 a. m. 

 Toxin administered by the mouth March 16, 1917, 10:30 a. m. 



The results in small animals, as outlined in the previous 

 charts, suggested that a relation might exist between B. botulinus 

 and the organism c-91, and more extensive tests were made upon 

 guinea pigs, with corroborative results. 



To further observe the relation between the organism 

 c-91 and the antitoxic serum prepared against B. botulinus, 

 horse No. 100 and mare 1001 were each given intravenously a 

 prophylactic dose of B. botulinus antitoxic serum at 11 :30 a. m. 

 on March 6th, 1917. At 4 p. m. the same day 2 cc. unfiltered 

 broth culture of c-91 were fed to these animals, mixed with 

 wholesome oats. At the same time two untreated horses, Nos. 

 98 and 99, each received 2 cc. unfiltered broth culture of the or- 

 ganism thoroly disguised in wholesome oats. Wholesome 

 feed was then supplied to these animals daily. At 6 o'clock 

 in the evening, March 6th, horse No. 100 was restless, refusing 

 to eat wholesome grain, nodding the head nervously, chewing 

 and salivating mildly. This animal was depressed and stupid 

 the fore part of the night, eating sparingly. No change was 

 observed on March 7th. On March 8th, at 7 a. m., horses Nos. 98 

 and 99 were permanently decumbent and neither could be in- 

 duced to stand. On the preceding day these animals had ap- 

 peared normal, eating the grain supplied at 5 o'clock in the 



