Studies in Forage Poisoning V. 77 



evening, but only a small amount of hay during the night. A 

 photograph was obtained of horses Nos. 98 and 99 at 10 a. m. 

 March 8th (Fig. 17). The tongue was paralyzed and protruded 

 in horse No. 98. Respirations were labored and accelerated in 

 both animals. Close observations were made with caution as 

 the feet were moved violently at intervals. At 3 p. m. horse 

 No. 98 died. On the morning of March 9th at 7 a. m. No. 99 

 was found dead. 



The lesions in horses Nos. 98 and 99 were strikingly similar 

 to the anatomical alterations in horses fatally stricken as the 

 result of ingesting broth cultures of B. botulinus, and also resem- 

 bled the alterations observed in forage poisoning. Cultures 

 were made from the blood and organs of Nos. 98 and 99. From 

 the caecum of horse No. 99 an anaerobic bacillus in association 

 with a coccus-like organism was regained which in broth culture 

 proved fatal to guinea pigs, per os. 



On March 8th, horse No. 100 was decumbent, but regained 

 a standing position with assistance. Antitoxic serum was ad- 

 ministered and the animal apparently gained in strength and 

 seemed stronger on March 9th. On this date the animal was 

 constipated and a sluggish condition was observed. At 2 o'clock 

 on March 10th, 1 grain arecoline and % grain strychnine were 

 injected subcutaneously. The above quantities placed in solu- 

 tion were prepared for four separate doses at intervals of four 

 hours, but by error were administered in one dose. It exerted 

 the usual effect, causing the animal to struggle and strain vigor- 

 ously the remainder of the afternoon. The following morning at 

 7 a. m. the animal was dead. At autopsy it was observed that 

 the pericardial sac was greatly distended with a large ante- 

 mortem blood clot completely surrounding the heart. In the 

 wall of the right auricle two distinct ruptures of the muscle wall 

 were found. Anatomic alterations in other organs were not ob- 

 served. 



On March 9th mare No. 1001 appeared nervous at intervals 

 and showed symptoms of mild colic. The appetite was not im- 

 paired and the animal remained apparently normal in health 

 and was released after thirty days. 



