78 Bulletin No. 207. 



In further tests of botulism antitoxic serum against the 

 organism c-91, three horses and one mule were employed. On 

 March 10th, 1917, at 10 :30 a. m., a sorrel horse, No. 1002, weight 

 940 pounds, a gray mare, No. 1003, weight 1,120 pounds, and 

 a bay horse, No. 1007, weight 870 pounds, received intraven- 

 ously varying amounts of clear botulism antitoxic goat se- 

 rum (H). On the following day, March llth, at 10 a. m., the 

 three treated horses and an untreated black mule, No. 105, 

 weight 810 pounds, received each 1 cc. of broth culture of c-91 

 organism thoroly mixed in 1000 grams of wholesome oats. 

 The feed was consumed immediately and a maintenance ration 

 of wholesome ear corn and mixed clover and timothy hay was 

 then provided each day. The water given these animals was 

 wholesome and from the supply furnished the live stock on the 

 Experiment Station farm. 



Beginning March 13th, mule No. 105 masticated awkwardly, 

 was slightly salivated, yawned frequently, appeared dull and 

 stupid, drank water slowly and apparently with some effort. 

 The appetite continued normal, and tho a marked stupor 

 prevailed, the symptoms observed were not augmented from 

 day to day as observed in previously infected animals., No 

 symptoms were observed in horses Nos. 1002, 1003, and 1004, 

 and at the expiration of eight days, on March 19th, at 9 a. m., 

 the serum horses in this experiment were injected intravenously 

 with botulism antitoxic sheep serum (C), and on the following 

 day were given 4 cc. of broth culture c-91 thoroly mix<td in 

 wholesome oats. The untreated mule, No. 105, which appeared 

 dull and stupid, was allowed an equal amount of the culture 

 (Fig. 18). The sleepy attitude of animal No. 105 is quite evi- 

 dent in Fig. 18. At 12 :30 p. m., on March 20th, No. 105 ap- 

 peared nervous, presenting symptoms of colic, slight salivation, 

 chewing, yawning, a jerking of the muscles in the region of the 

 flank, shoulders, face and lips. On March 21st at 7 a. m., No. 

 105 was permanently decumbent, the tongue was paralyzed and 

 pendulous. Fig. 19 was photographed at 10:30 a. m. on March 

 21st and at 10 :45 mule No. 105 died. 



The gross anatomic alterations observed in mule No. 105 

 at autopsy were as follows : Tongue protruding ; no external 



