Studies in ^Forage Poisoning V. 79 



abrasions. Subcutaneous and muscular tissues appeared nor- 

 mal. The nasal and mouth cavities, with the exception of the 

 tongue previously mentioned, appeared normal. Meninges 

 mildly injected. Lungs congested ; pleura injected and ramified. 

 Heart showed numerous hemorrhages on the wall. Stomach 

 slightly inflated with gas; outer wall injected. Outer wall of 

 small intestine injected; a sticky mucus adhered to the inner 

 wall ; scattered punctate hemorrhages with ramification and in- 

 jection merging into localized areas of hemorrhagic enteritis on 

 inner wall (Fig. 20). A few mesenteric lymph nodes swollen 

 and mildly congested. Diffuse congestion and numerous hem- 

 orrhages on inner wall of caecum. Capsule of kidney easily re- 

 moved; cortex and medulla highly colored and easily disrupted 

 on pressure. In the other organs and tissue no anatomic alter- 

 ations were visible. 



Plantings in alkaline pork broth were made of the organs 

 and incubated under anaerobic conditions. From the stomach 

 of this animal an anaerobic bacillus resembling the organism 

 ingested by this animal was isolated which proved fatal when 

 feel to guinea pigs. 0.5 cc. broth culture administered by the 

 mouth to a guinea pig at 9 :30 a. m. on March 30th, 1917, re- 

 sulted in death on March 31st at 3 :30 p. m. Fig. 21 illustrates 

 the prostrate condition of the animal in 20 hours subsequently to 

 ingesting the organism isolated from the stomach contents of 

 mule No. 105. 



r 'ne antitoxic serum apparently afforded protection in 

 horses Nos. 1002, 1003, and 1004. The 1 cc. of the broth culture 

 of c-91 administered on March llth was followed by stupor 

 and weakness in mule No. 105, while no effect could be observed 

 in horses Nos. 1002, 1003, and 1004, nor was any manifest dis- 

 turbance observed in these horses following ingestion of 4 cc. of 

 the organism subsequently to injections of antitoxic sera. The 

 symptoms observed in No. 105 after receiving 1 cc. unfiltered 

 broth culture were not as pronounced nor as acute as observed in 

 other experimentally infected animals. In one other animal sim- 

 ilarly infected the subacute type of infection was observed, 

 accompanied by prolonged stupor and cachexia, eventually ter- 

 minating in death. 



