84 Bulletin No. 207. 



further extend observations in this connection, No. 107, a bay 

 mare, weight 900 pounds, No. 112, a bay mare, weight, 1,040 

 pounds, and No. 1012, a blind bay mare, weight 1,020 pounds, 

 each received 2 cc. broth culture of go-6 in 1000 gms. of whole- 

 some oats on March 21st, 1917, at 11 a. m. (Fig 27). Horse 

 No. 1012 received a protective dose of botulism antitoxin 24 

 hours before the ingestion of the broth culture go-6. The con- 

 trols, Nos. 107 and 112, did not receive antitoxin. Wholesome 

 feed was then supplied to these animals each day. On March 

 24th, horse No. 107 was dull and depressed, prehended feed awk- 

 wardly and slowly, held it in the mouth or dropped it after par- 

 tial mastication. At intervals salivation was marked (Figs. 28 

 and 29). Muscular weakness developed rapidly and on March 

 25th this animal was permanently decumbent, while horses Nos. 

 112 and 1012 appeared normal (Fig. 30). On March 26th a 

 profuse hypersecretion of mucus from the mouth was observed 

 in horse No. 107 thruout the day; the tongue was paralyzed 

 and protruded; an audible clicking sound in throat; enuresis; 

 death followed during the night. The gross changes observed 

 in No. 107 at 8 a. m. March 28th consisted of injection of 

 meninges, congestion of lungs, ecchymoses on heart wall, injec- 

 tion of mucosa of small intestine, with localized hemorrhagic 

 areas. A sticky mucus adhered to inner wall of the small in- 

 testine; the duodenal portion of the mesentery was ramified. 

 Kidney soft and friable. Other gross changes were not ob- 

 served. 



No. 112 showed no discomfort until March 30th, at which 

 time the feed was prehended slowly and with some difficulty. 

 The appetite was apparently normal. Similar symptoms were 

 observed on March 31st, April 1st, 2nd, and 3rd, and on the 

 4th a marked paresis of the pharynx was observed. Attempts 

 to drink were continuous, but water was returned thru the 

 nasal passages. There was a contracted appearance in the 

 flank and a noticeable cachexia. At 10 a. m. on April 2nd 

 corn was prehended, masticated and dropped from the open 

 mouth (Fig. 31) and the inferior maxilla was moved laterally. 

 These symptoms were rapidly augmented from this date, ac- 

 companied by marasmus, and on April 5th the animal was per- 



