Studies in Forage Poisoning V. 85 



manently decumbent (Fig. 32). No effort to prehend or masti- 

 cate feed while in a decumbent state was observed. The animal 

 frequently neighed on the approach of the attendant and ex- 

 hibited a noticeable olophonia. Death occurred during the night 

 of April 5th. The gross anatomic lesions observed at 8 a. m. 

 April 6th were as follows : Ecchymoses in larynx and pharynx ; 

 congestion of lungs and pleural fibrous filaments and pleural 

 ecchymoses. Outer wall of stomach ramified; two localized 

 areas of injection and punctate hemorrhages on inner wall of 

 small intestine ; contents of small intestine watery and a mucus- 

 like substance adhered to the inner wall. Inner wall of caecum 

 showed ecchymoses and the colon showed similar tho more 

 extensive ecchymoses. 



On April 6th horse No. 1012 was eating and to all appear- 

 ances normal. A transitory reaction was observed on March 

 30th, at which time antitoxic serum was administered intraven- 

 ously. The external manifestations soon subsided and the animal 

 remained apparently normal and was released on April 20th. 



The Serological Relation of Botulism Antitoxic Serum to the 



Culture Filtrate of an Organism from an Oat Hay. 

 The effect of the unfiltered broth culture of the organism 

 go-6 upon guinea pigs and horses, and its apparent relation to B. 

 botulinus as observed culturally and morphologically and also 

 in the protection afforded by serum immune to B. botulinus 

 against artificial infection by the unfiltered broth cultures, sug- 

 gested the administration of the sterile filtrate of this organism 

 to horses in combination with antitoxic serum. For this pur- 

 pose. No. 1013, a blind bay horse, weight 640 pounds, No. 1014, 

 a sorrel pony, weight 650 pounds, No. 1015, a blind dun pony, 

 weight 660 pounds, and No. 1016, a blind bay mare, weight 

 840 pounds, received intravenously a prophylactic dose of anti- 

 toxic goat serum (I) on March 27th, 1917, at 11 :30 a. m. The fol- 

 lowing day at 11:30 a. m. these four serum treated horses re- 

 ceived each 2 cc. filtered (Pasteur-Chamberland B) broth cul- 

 ture of the organism go-6 in 1000 gms. of wholesome oats. Si- 

 multaneously No. 110, a bay horse, weight 850 pounds, No. 109, 

 a bay horse, weight 990 pounds, and No. 114, a brown mare, 



