88 Bulletin No. 207. 



intestine was highly injected and accompanied by numerous 

 small hemorrhages (Fig. 36). 



Horse No. 113 was less acutely afflicted, but succumbed 

 April 7th. Horse No. 115 received 2 cc. of broth culture of 

 the organism go-6, isolated from the oat hay water, in whole- 

 some oats, on April 6th and succumbed April llth. The clin- 

 ical symptoms and anatomic changes observed in Nos. 113 and 

 115 were somewhat similar to recorded observations in other 

 horses similarly infected. The drinking of the oat hay water 

 for a period of thirty days, as observed in horses Nos. 108, 113 

 and 115, apparently did not establish a tolerance against the 

 ingestion of 2 cc. broth culture of the pathogenic organism iso- 

 lated from the water in which the oat hay was immersed. 



In conjunction with the horses receiving oat hay water and 

 wholesome feed as outlined, beginning February 13th, 1917, 

 a gray pony, No. 1050, and a sorrel horse, No. 1051, received 

 water from the barrel in which the oat hay was immersed and 

 also weekly subcutaneous injections of serum immune to B. 

 botulinus. No. 1050 consumed 180 gallons of the oat hay water, 

 a daily average of 6 gallons; No. 1051 consumed 151% gallons, 

 a daily average of 5+ gallons. At the end of 30 days, March 

 13th, these animals received 2 cc. of broth culture of the or- 

 ganism (go-6) isolated from the oat hay water disguised in 

 wholesome feed. No noticeable effect as the result of consuming 

 the water or the organism was observed in these animals. A 

 black mule, No. 106, was allowed to drink water from the barrel 

 in which the oat hay was immersed from February 15th to 

 March 15th. 160% gallons of water were consumed during this 

 time, or 5+ gallons per day. On February 14th a subcutaneous 

 injection of antitoxic serum was given and repeated each week 

 until March 15th, when wholesome water was substituted for the 

 oat hay water and 2 cc. broth culture of the organism (go-6) 

 isolated from the oat hay water was fed to this animal, disguised 

 in wholesome feed. On March 20th, and 21st, there was ob- 

 served in this animal a discharge from the nostrils, increased 

 and labored respiration, temperature of 102.4 F. Death oc- 

 curred March 22nd. Post-mortem examination showed pro- 

 nounced croupous pneumonia involving both lungs. 



