Studies in Forage Poisoning V. 89 



Beginning February 21st, a bay horse, No. 1052, and a brown 

 mule, No. 1053, were injected weekly with botulism antitoxin 

 and allowed wholesome feed and water from the barrel 

 in which the oat hay was immersed. No manifest symptoms 

 were observed in these animals. No. 1052 consumed 165 gallons, 

 or an average of 5+ gallons per day. No. 1053 consumed ap- 

 proximately 166 gallons of the oat hay water, or an average of 

 5+ gallons per day. Immediately following the ingestion of the 

 oat hay water 2 cc. broth culture of the organism (go-6) 

 were consumed by horses Nos. 1052 and 1053 without noticeable 

 effect. On Februar}' 27th, a bay stallion, No. 1054, and a bay 

 mare. No. 97, were allowed wholesome feed and water from the 

 barrel in which the oat hay was immersed. On February 26th, 

 Nos. 97 and 1054 had each received a subcutaneous injection of 

 serum immune to B. botulinus. No. 97 succumbed on March 

 3rd after manifesting characteristic symptoms of forage poison- 

 ing. The anatomic changes observed were likewise suggestive 

 of this disease. Each week thereafter No. 1054 received sub- 

 cutaneous injections of the antitoxic serum, and received the 

 oat hay water for a period of 30 days, during which time 121% 

 gallons were consumed, or a daily average of 4+ gallons. This 

 animal then received wholesome water and 2 cc. broth culture 

 of the pathogenic organism isolated from the oat hay water, 

 disguised in wholesome oats. The animal remained apparently 

 healthy and was released after a period of thirty days. 



On March 6th, a gray mare, No. 1055, and a sorrel horse, 

 No. 1056, were allowed water from the barrel in which the 

 oat hay was immersed, supplemented by weekly subcutaneous 

 injections of antitoxic serum. During this time Nos. 1055 and 

 1056 each consumed approximately 171 gallons of the water, 

 or a daily average of 5+ gallons. On April 6th, each received 

 2 cc. broth culture of the pathogenic organism isolated from the 

 oat hay water disguised in wholesome feed. No symptoms were 

 observed in these animals and they remained apparently healthy. 



The horses drinking the water in which the oat hay was 

 immersed furnished evidence that a water soluble product was 

 primarily responsible for the death of horses Nos. 95, 96, 97, 

 and 102. Horses Nos. 108, 113, and 115 were not affected by 



