124 



Bulletin No. 208. 



The Relation of Botulism Antitoxin to the Organism 

 Isolated from the Ensilage. 



Three guinea pigs, weighing from 550 to 900 grams each, 

 were given intraperitoneally 0.5 to 1.5 cc. botulism* antitoxin, 

 followed in 24 hours by many times the fatal amount, per os, of 

 the organism isolated from the ensilage, in broth culture, without 

 noticeable effect. A similar amount of the broth culture was 

 given at the same time to an untreated pig, followed by death 

 in approximately 20 hours (Chart 1). Duplicate tests upon 

 guinea pigs were conducted, as indicated in Chart 2 and Fig. 

 3. 



CHART 1. 



CHART 2. 



The effect of the filtered broth culture of the organism iso- 

 lated from the ensilage, administered to guinea pigs in a similar- 

 manner in conjunction with serum immune to B. botulinus, is 

 presented in Chart 3 and Fig. 4. The sterility of the filtrate 

 was demonstrated by cultural methods. 



*B. botulinus (N. B. S. strain) obtained from Dr. Buckley, Bureau of 

 Animal Industry, Washington, D. C. 



