Studies in Forage Poisoning VI. 



125 



CHART 3. 



The protective quality of serum immune to the organism 

 isolated from the ensilage, as related to B. botulinus, is illus- 

 trated in Chart 4 and Fig. 5. 



CHART 4. 



The apparent relation of B. botulinus (N. B. S. strain) 

 to the organism isolated from the silage was further observed 

 in three horses and one mule. On May 15th, 1917, at 11 a. m., 

 horse No. 1019, a bay mare, weight '750 pounds; horse No: 

 1020, a bay gelding, weight 750 pounds, and horse No. 1021, a 

 brown mare, weight 680 pounds, were each given an intraven- 

 ous injection of botulism antitoxin. On May 16th at 11 a. m. 

 the three horses, Nos. 1019, 1020 and 1021, and a control mule 

 No. 118, weight 900 pounds, each received 2 cc. broth culture 

 of the organism isolated from the ensilage, in 1000 grams of 

 wholesome oats fed in individual enamel pans (Fig 6). 



These animals were then fed wholesome corn, shelled oats, 



