Studies in Forage Poisoning VI. 



129 



Agglutination Test of Organism Resembling 1 B. botulinus,* 

 Isolated irom the Ensilage 



,T 7. 



* B. botulinus (X. B. S. strain) from Buckley of Bureau of Animal In- 

 dustry, AVash. 



** Serurn from horse fatally infected with B. botulinus. 

 *** Serum from horse fatally infected with anaerobic organism from en- 

 silage. 



{-Positive Reaction Negative Reaction 



Agglutination Test of Anaerobic Organism Resembling 

 B. botulinus, Isolated from the Ensilage. 



CHART 8. 



Agglutination Test of Anaerobic Bacillus Resembling B. botulinus, 

 Isolated from Horse Fatally Afflicted from Drinking Oat 

 Hay Water 

 CHART 9. 



+ Positive Reaction Neg-ative Reaction 



* Anaerobic bacillus isolated from horse fatally afflicted from drinking 

 oat hay water. 



** Anaerobic bacillus isolated from Games' ensilag-e. 



** Serum from horse in moribund condition following- the ing-estion of 2 

 cc. broth culture of organism resembling- B. botulinus isolated from an 

 oat hay. 



**** Serum from mule in moribund condition following- the ing-estion of 3 

 cc. broth filtrate of organism isolated from Games' ensilage. 



