CONTENTS. 



Page. 



General plan of the investigation 9 



Information furnished by cold-storage warehousemen 11 



Inception of the work 25 



I. Cold-storage eggs : 27 



Plan of work 27 



Physical characteristics .-. ! 28 



Chemical analysis 29 



Discussion of data . 29 



Conclusions 30 



Bacteriological examination 34 



Technique 34 



Discussion of results . g 34 



Microscopical examination 36 



Fresh eggs 36 



Cold-storage eggs. . , .- 36 



Supplementary work on rosette crystals 37 



II. Quail cold-stored under known conditions 37 



Organoleptic tests ; 37 



Bacteriological examination 40 



III. Chickens cold -stored under known conditions 44 



Organoleptic tests 44 



Bacteriological examination 51 



IV. Market cold-storage chickens ; 57 



Introduction 57 



Chemical studies 59 



Resume of the literature on the chemical composition of poultry. . . 59 



Selection of material for study 61 



Chemical technique 62 



Preparation of sample 62 



Muscle determinations 63 



Total solids and water _" 63 



Fat 63 



Ash 63 



Nitrogenous constituents 64 



Fat determinations 66 



Analyses of fresh chickens 67 



Analyses of cold-stored chickens 69 



Bacteriological studies 75 



Technique : 75 



Examination of fresh and cold-stored chickens 77 



Histological studies 79 



Technique 79 



Examination of fresh and cold-storage chicken muscle 81 



5 



