BAIRD c^TATLOCK (LONDON) LTD. 



Microscopic Stains. 



(BURROUGHS, WELLCOME & Co.) Trade Mark " Soloid " Brand. 



The word " Soloid " is a brand which designates fine products manufactured by Burroughs, Wellcome & Co. 

 To ensure the supply of these pure and reliable preparations this brand should always be specified when ordering. 



The tendency of solutions of the aniline dyes to decompose has always been a source of trouble in micro- 

 scopic work. The " Soloid " Microscopic Stains enable solutions to be prepared in small quantities when required. 



List of " Soloid " Brand Microscopic Stains, in tubes containing six tabloids. 



" SOLOID " Brand- 



BISMARCK BROWN, pure, o.i gm. 

 BORAX METHYLENE BLUE 



EHRLICH'S TRIPLE STAIN 



EOSIN, pure, o.i gm. 



EOSIN-METHYLENE BLUE, 0.05 gm. (Louis 



Jenner) 



EOSIN AZUR., for Giemsa's Staining with one 



solution, 0.038 gm. 

 FUCHSINE, pure, o.i gm. 

 GENTIAN VIOLET, pure, o.i gm. 

 GRAM'S IODINE SOLUTION, 15 c.c. 



Per doz. tubes. 

 s. d. 

 80 

 80 

 10 o 



Per doz. tubes. 



8 o 



18 



8 



SOIOID " Brand 



HVMATOXYLIN, pure, o.i gm. . . 



HYMATOXYLIN (Delafield) 



METHYLENE BLUE, pure, o.i gm. 



METHYL VIOLET, pure o.i gm. 



SODIUM CARBONATE, .05 gm. 



THIONIN BLUE, pure, o.i gm 



TOISON'S BLOOD FLUID 



ROMANOWSKY STAIN (Leishman's Powder] 



0.015 m - 

 ROMANOWSKY STAIN (Wright's Modification] 



0.05 gm. 



d. 

 o 

 o 

 o 

 o 

 o 

 o 

 o 



DIRECTIONS FOR USE. 



The amounts of distilled water and absolute alcohol respectively required to produce saturated solutions of certain dyes in 

 common use are indicated in the following table. In all cases the " Soloid " product should be thoroughly powdered before it 

 is shaken up in the solvent. 



Aqueous dilutions of the above, containing 5 to 10 per cent, of these saturated solutions, are well adapted for ordinary stain- 

 ing purposes. Various other solutions, ready for immediate use, may be prepared from " Soloid " Microscopic Stains according 

 to the following directions : 



Eosin. To obtain a solution of eosin suitable for general staining, one " Soloid " product may be dissolved in 20 c.c. of 

 50 per cent, pure alcohol. This gives a 0.5 per cent, solution. 



Loefiler's Alkaline Methylene Blue. Dissolve one " Soloid " Methylene Blue in 7 c.c. of absolute alcohol, and add 25 c.c. 

 of distilled water to which one drop of Liquor Potassae P.B. has been added. 



Aniline Gentian Violet. Dissolve one " Soloid " Gentian Violet in 7 c.c. of absolute alcohol ; and add 63 c.c. of a freshly 

 filtered saturated solution of aniline oil in distilled water. 



Zeibl's Carbol Fuchsine. Thoroughly powder one " Soloid " Fuchsine in 3 c.c. absolute alcohol ; add 22 c.c. of 5 per cent, 

 aqueous solution of carbolic acid ; shake well and filter before using. 



Gram's Iodine Solution. Dissolve one " Soloid " product of reagent A in 10 c.c. of distilled water ; add one of reagent B, 

 and when solution is complete, dilute to 15 c.c. with distilled water. 



Carbol Thionin Blue. Dissolve one " Soloid " Thionin Blue in 100 c.c. of a 5 per cent, aqueous solution of carbolic acid. 



Borax Methylene Blue. Dissolve one " Soloid " Borax Methylene Blue in 10 c.c. of distilled water. 



Delafield - s Haematoxylin. Dissolve one " Soloid " Haematoxylin (Delafield) in 10 c.c. of a 25 per cent, solution of glycerin 

 in water. 



Eosin-methylene Blue (Louis Jenner's Stain). Dissolve one " Soloid " product in 10 c.c. of pure methyl alcohol. 



Romanowsky's Stain (Leishman's Modification). Dissolve one " Soloid " product in 10 c.c. of purr methyl alcohol. 



In the cases of Loeffler Alkaline Methylene Blue, Aniline Gentian Violet, and Zeihl's Carbol Fuchsine, the solutions obtained 

 as above are only approximately equivalent to those prepared according to the original formula. The figures avoid small fractions 

 in measurement ; this enables the solution to be prepared more readily without diminishing the efficiency of the stain. 



.CHEMICAL AND SCIENTIFIC APPARATUS AND PURE CHEMICALS. 





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