304 URINE. [CH. XII. 



369. Roberts 9 test. Repeat the previous exercise, using 

 Roberts' reagent in place of the nitric acid. A white ring at the 

 junction of the fluids indicates albumin. 



NOTES. i. Roberts' reagent is prepared by adding I volume of pure 

 nitric acid to 5 volumes of a saturated solution of magnesium sulphate. 



2. Coloured rings are not formed, and so confusion is avoided. 



370. Spiegler's test. Render the urine faintly acid with 

 acetic acid and repeat the above test, using Spiegler's reagent in 

 place of Roberts*. A white ring indicates the presence of albumin. 



NOTES. i. Spiegler's reagent consists of 

 Mercuric chloride 

 Tartaric acid 

 Glycerine 

 Sodium chloride 

 Distilled water .. 



40 grams. 



20 grams. 



100 grams. 



50 grams. 



1000 cc. 



2. The reaction is also given by albumoses and peptones. 



3. The test serves to show i part of albumin in 250,000. It is almost too 

 delicate for ordinary clinical work, as a large number of apparently normal 

 urines give a positive reaction. 



371. Sulphosalicylic test. To a few cc. of the clear, filtered 

 urine add a large " knife point " or a few drops of a 20 per cent, 

 solution of sulphosalicylic acid (see Ex. 18). A cloud or precipitate 

 indicates the presence of albumin. 



2. Albumoses. 



Albumoses are found in the urine in certain cases of 

 degeneration of the intestinal epithelium ("alimentary 

 albumosuria"). Also in a variety of other conditions such 

 as in the absorption of pneumonic exudates, in some cases 

 of an increased breakdown of the tissues in certain fevers, 

 in the puerperium and in urine containing semen. 



The albumose present seems to be a secondary album ose. 



372. Remove any albumin that may be present by heat 

 coagulation. To the filtrate apply Spiegler's test (Ex. 280). A 

 white ring indicates the presence of albumose. 



j. Bence- Jones* Protein. 



In certain cases of disease of the bone marrow (multiple 

 myeloma), and possibly in osteomalacia, a protein with 



