14 APPARATUS 



Platinum Loops. For use in making loops and needles, platinum wire of 26 gauge 

 will be found most suitable. The handle made of glass rod is preferable to the 

 metal ones. One end is fused in the flame and, holding the 3- to 4-in. piece of plati- 

 num wire, with forceps, in the same flame, insert the glowing metal into the molten 

 glass. By taking two lengths of platinum wire and twisting them together a more 

 rigid needle is made for inoculating stab cultures. 



Isolation cf colonies uslny two halves cf 

 ayar plate Instead cf two separate plates. 



Zlnsser's anaerobic 

 plate, method 



gutter's vaccine 

 ampule filler 



FIG. 6. i, Method of using one plate instead of two for isolation of colonies, 

 (see page 50). The separated colonies on the No. II side of the plate are studied 

 with unaided eye and aplanatic triplet (^ in. or % in.) both by reflected and trans- 

 mitted light. After we have determined the presence of two or more different 

 kinds of colonies, a well separated one of each type is selected and a blue pencil ring 

 made around it on the glass surface of the back of the Petri dish together with a num- 

 ber. This number is carried along on culture tubes or microscopical slide prepara- 

 tions until the organism is identified. 2, Lyon tube for blood. More convenient 

 than the Wright tube. For description see page 18. 3, Bronfenbrenner's an- 

 aerobic tube. This is made by drawing out a single test-tube instead of using two 

 separate tubes as with the Noguchi method. Before drawing out the test-tube the 



Eiece of sterile tissue is introduced and after drawing out in the flame the ascitic 

 roth or sheep serum water, as well as the material for culturing, is introduced 

 through the drawn-out neck with a bulb capillary pipette. The lower part of the 

 tube is placed in water at 37C. and the vacuum connection made and after exhaus- 

 tion of air the sterile paraffin oil is run in. The drawn-out portion is then sealed off 

 in a small flame and looks like a sealed vaccine ampule. 4, Noguchi apparatus. 

 5, Zinsser method for anaerobic plates (page 80). 6, Butler's apparatus for filling 

 vaccine ampules. The sterile vaccine is put in the sterile flask, and the stopper with 

 air intake and needle filler separately sterilized and then introduced into neck of 

 flask which is then inverted. 



A platinum loop made around a piece of copper wire, 4 mm. in diameter holds 

 about 2 mg. of culture taken up from an agar slant. Kolle estimates that an 

 agar slant of typhoid bacilli or of staphylococci should contain 15 such loopfuls 



