CONTENTS 



PART I. BACTERIOLOGY 



CHAPTER I. APPARATUS. 



The Microscope, i Apparatus for sterilization, 6 Cleaning glass- 

 ware, 9 Concave slides, fermentation tubes, n. 



CHAPTER II. CULTURE MEDIA. 



Nutrient bouillon, 20 Standardization of reaction, 21 Sugar-free 

 bouillon, 23 Glycerine bouillon, 25 Peptone solution, 25 Nutrient 

 agar, 26 Glycerine agar egg medium, 27 Gelatine, 28 Litmus milk, 

 28 Potato, 29 Blood serum, 30 Blood agar, 31 Bile and faeces 

 media, 31-33 Culture media for protozoa, 35. 



CHAPTER III. STAINING METHODS. 



Loffler's methylene blue, 39 Carbol fuchsin, 39 Gram's method, 39 

 Acid-fast staining, 41 Neisser's stain, 42 Capsule staining, 43 

 Flagella staining, 44 Spore staining, 45 Staining of protozoa, 45. 



CHAPTER IV. STUDY AND IDENTIFICATION OF BACTERIA. GENERAL CON- 

 SIDERATIONS. 

 Methods of isolating bacteria, 48 Classification, 50 Use of keys, 51. 



CHAPTER V. STUDY AND IDENTIFICATION OF BACTERIA. Cocci. 



Key, 57 Streptococci, 58 Sarcinae, 62 Staphylococci, 63 Pneu- 

 mococcus, 64 Gram-negative cocci, 66. 



CHAPTER VI. STUDY AND IDENTIFICATION OF BACTERIA. SPORE-BEARING 



BACILLI. 



Key, 73 Anthrax, 75 Cultivation of anaerobes, 78 Malignant 

 cedema, 82 B. botulinus, 82 B. tetani, 84 B. aerogenes capsulatus, 



87- 



CHAPTER VII. STUDY AND IDENTIFICATION OF BACTERIA. BRANCHING, 

 CURVING BACILLI. MYCOBACTERIA. CORNYEBACTERIA. 



Acid-fast bacilli, 91 Tubercle bacillus, 92 Leprosy bacillus, 97 



Non-acid-fast branching bacilli, 101 B. mallei, 101 B. diphtherias, 



102 Hofmann's bacillus, 107 B. xerosis, 108. 

 CHAPTER VIII. STUDY AND IDENTIFICATION OF BACTERIA. 



Gram-negative bacilli, Hemophilic bacteria, in Influenza bacillus, 



in Priedlander's bacillus, 114 Plague, 114 Eberth, Gartner, and 



Escherich groups, 118 Typhoid, 119 Dysentery, 125 Chromogenic 



bacilli, 129. 

 CHAPTER IX. STUDY AND IDENTIFICATION OF BACTERIA. 



Spirilla, 131 Cholera, 131. 



CHAPTER X. STUDY AND IDENTIFICATION OF MOULDS, 136. 

 CHAPTER XI. BACTERIOLOGY OF WATER, AIR, AND MILK. 



Water, 149 Milk, 155 Air, 159. 



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