74 



STUDY AND IDENTIFICATION OF BACTERIA 



NONPATHOGENIC SPORE-BEARING AEROBES ON AGAR 

 Modification of table of Gruner and Fraser. 



Gray white 



B. subtilis (motile). 



B. ellenbachensis (non-motile). 



B. cretaceus. 



B. mycoides. 



B. ochraceus. 



B. mesentericus. 



B. ruminatus. 



B. graveolens,. 



B. vulgatus. 



NOTES. B. subtilis has square ends and central spores, not causing bulging. 



B. megatherium has spore toward one pole which may be terminal. 



B. vulgatus is long and slender. Slightly oval spores. 



B. mesentericus varies. Usually short with rounded ends and central bulging 

 spores. 



B. ellenbachensis has rounded ends, oval spores and shows granule formation 

 (beaded) resembling diphtheroids. 



All of these grow well at room temperature, optimum 3oC. 

 B. Grow only anaerobically. 



1. Rods very little swollen by centrally situated spores, 

 (a) Motile. B. cedematis maligni. (Gram-negative.) 

 (6) Nonmotile. B. aerogenes capsulatus. (Capsule.) 



2. Spores tend to be situated between center and end. 

 (a) No liquefaction of gelatin. B. butyricus. 



(6) Gelatin liquefied slowly. 



B. botulinus. Milk not coagulated. 



B. anthracis symptomatici. 



B. enteritidis sporogenes. Milk coagulated with abundant gas. 

 (c) Gelatin liquefied rapidly. B. cadaveris sporogenes. Very motile. 



3. Spores situated at end of rod. Drum-stick sporulation. TETANUS GROUP. 



The following table taken from Lehmann and Neumann, based on 

 pathogenic effects, is of great practical value. After inoculation of 

 some animal subcutaneously with the suspected material we have: 



A. No particular symptoms at site of inoculation. 



Absorption of the soluble toxin causing: 



(1) General symptoms of tetanus. B. tetani. 



(2) Botulism poisoning symptoms. Pupillary symptoms. Paralysis of 

 tongue and pharynx. Cardiac and respiratory failure. 



B. Local symptoms marked at site of inoculation. Hemorrhagic emphysema tous 

 oedema. 



(i) Motile. 



(a) Gram-negative. B. cedematis maligni. 



(6) Gram-positive. ^anthracis symptomatici. 



