i8o 



PRACTICAL METHODS IN IMMUNITY 



Pure lipoidal substances as contained in Noguchi's acetone insoluble antigen, 

 however, do not act in this way. 



Consequently by using such an antigen we eliminate the objection to employing 

 fresh human serum in the test for syphilitic antibodies. 



As giving more uniform haemolytic results and as being more stable and easier 

 of employment, I have made use of Noguchi's directions for taking up the serum of 

 the rabbits, immunized to human red cells and his method of standardizing this 

 "amboceptor" paper. In practice, I measure off the length of paper correspond- 



CONTROL 



U. 40 I tobO 

 ANTIGEN ANTIGEN ANTIGEN ANTIGEN ANTI&EN ANTIGEN 



5 



FIG. 49. i. Capillary pipette being graduated by drawing up i and 4 drops 

 from a watch-glass, (a) Blue pencil mark of i drop or i volume, (b) Mark of 

 volume of 4 drops. 2. Graduated centrifuge tube containing sodium citrate normal 

 salt solution. 3. Tube with 10 amboceptor units in i c.c. of salt solution. 4. Mix- 

 ture of i volume 20% emulsion red cells and 4 volumes inactivated amboceptor 

 solution. 5. Small glass tubes for Emery test. 6. Method of transferring from 

 tube to tube. 7. Making a Wright U-tube the end "a" to be used as a capillary 

 pipette. 



ing to 10 Noguchi units and dissolve the dried serum in such paper in i c.c. of salt 

 solution. This make a satisfactory and uniform substitute for the sterile immune 

 serum used by Emery. 



Method: i. Take blood from the finger or ear in a large Wright U tube (Y inch 

 in diameter). Place in 37C. incubator for fifteen minutes (to increase yield of 

 serum) and then centrifuge. 



Of course wheli the Noguchi test is being made at the same time we 

 use the serum of the blood taken from a vein in the arm. 



