214 MICROMETRY AND BLOOD PREPARATIONS 



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tis- 



alcohol. Next dissolve 25 grams of ammonia alum in 500 c.c. of distilled wat 

 Mix the two solutions and allow to ripen for a few days. The stain should be satis- 

 factory in two or three days. 



To make Dela field's haematoxylin, dissolve i gram of haematoxylin crystals in 

 6 c.c. of 95% alcohol. Add this to 100 c.c. of saturated aqueous solution of ammonia 

 alum. After exposure to light for a week, the color changes to a deep blue purple. 

 Add to this ripened stain 25 c.c. of glycerine and 25 c.c. of methyl-alcohol and, after 

 it has stood for about two days, filter. The stain should be filtered from time to time 

 as a sediment forms. This makes a stock solution which should be diluted 10 to 15 

 times with water when staining. 



Mink's Modification of Unna's Haematoxylin 



Haematoxylin i gram 



Alum 8 grams. 



Sulphur (sublimed) i gram. 



Glycerine 30 c.c. 



Alcohol 50 c.c. 



Water 100 c.c. 



Dissolve the haematoxylin in the glycerine in a mortar. Dissolve the alum in the 

 water and add it to the glycerine hsematoxylin in the mortar. Then add the sulphur 

 and the alcohol. The solution ripens in about three to four days. Allow the sedi- 

 ment to remain in the bottom of the bottle containing the stain and filter off small 

 quantities as needed. 



3. Wash for two to five minutes in tap water to develop the haematoxylin color. 



4. Stain either with a i to 1000 aqueous solution of eosin or with a one-half of i % 

 eosin solution in 70% alcohol. The eosin staining only requires fifteen to thirty 

 seconds. 



5. Wash and examine. 



IODOPH3LIA 



This reaction is supposed to be due to the presence of glycogen, 

 especially in the polymorphonuclears, in suppurative conditions. 



It has been stated that a differentiation between the joint involvement in gonor- 

 rhceal infection and in articular rheumatism may be made from iodophilia being 

 present in the gonococcus infection. 



Make blood-smears on cover-glasses as usual, and after they dry, but without 

 fixation, mount them in a drop of the following solution: 



Iodine i part. 



Potassium iodide 3 parts. 



Gum arabic 50 parts. 



Water 100 parts. 



Small brown masses in the polymorphonuclears indicate a positive iodophilia. 

 Viscosity of the Blood. This is estimated by observing the relative height to which 

 blood rises in capillary tubes as compared with water, and normally varies from three 



