36 



Castellani and Chalmers' Grouping. These authors have recently 

 suggested a rather unique classification of bacteria in which the organisms 

 generally considered as members of the colon group are distributed among 

 three Tribes and three Genra as follows: 



Tribe Encapsulateae. (Castellani and Chalmers, 1918. Bacillaceae 

 growing well on ordinary media, without endospores; neither fluorescent 

 nor chromdgenic; aerobes; facultative anaerobes; not liquefying gelatin; 

 possessing capsules in animal tissue. 



The genus Encapsulates includes two lactose fermenting (acid and gas) 

 species differentiated on fermentation of inosite. 



Inosite not fermented. 1. Encapsulates acidi lactici 



Inosite fermented with acid and gas. 2. Encapsculatus lactis aerogenes 



Tribe Proteae. (Castellani and Chalmers, 1918) Bacillaceae grow- 

 ing well on ordinary media; without endospores; aerobes; neither fluorescent 

 nor chromogenic; aerobes; facultative anaerobes; not 1 iquefying gelatin, 



They recognize two lactose fermenting species in the genus Cloaca 

 which are different on sucrose fermentation. 



Sucrose fermented with acid and gas. 1. Cloaca cloacae. 



Sucrose not fermented 2. Cloaca levcns. 



All other members of the colon group are placed in their Genus Escher- 

 ichia of the Tribe Ebertheae. 



Tribe Ebertheae. (Castellani and Chalmers, 1918). Bacillaceae 

 growing well on ordinary laboratory media; not forming endospores; aer- 

 obes, and often facultative anaerobes; without fluorescence pigment forma- 

 tion or gelatin liquefaction; without polar staining; Gram negative; with- 

 out a capsule. 



Genus Escherichia. (Castellani and Chalmers, 1918.) Ebertheae 

 which ferment glucose and lactose completely with acid and gas; milk 

 clotted. 

 I. Indol positive division. (Smith) 



A. Sucrose fermented Communior section of Durham. 



1. Dulcitol fermented with acid and gas. 



a. Adonitol fermented with acid and gas. 



(1) Motile 1. Escherichia oxytocus 



(2) Non motile. 2. Escherichia metacoli 



b. Adonitol no change. 



(1) Motile. 



(a) Agglutinated by pseudocoli serum 



3. Escherichia pseudo-coli 



(b) Not agglutinated (late fermentation of sucrose) 



4. Escherichia coliformis 



(2) Non motile. 5. Escherichia neapolitanus 



2. Dulcitol not fermented, 

 a. Motile 



(1) Inosite fermented with acid and gas. 



6. Escherichia pseudocoloidella 



(2) Inosite no change 7. Escherichia pseudocoloides 



