74 



high ratio type (aerogenes section), and 7 (4.2%) produced only hydrogen. 

 They noted further that none of the types found on grains were identical 

 with those obtained by them from bovine feces, the low ratio types from 

 grains being pigmented. 



Stokes isolated 77 strains from corn meal, grape nuts, post toasties, 

 corn flakes, puffed wheat, quaker oats, rye, corn, wheat, barley, and oats, 

 of which 57 (74.0%) were of the aerogenes cloacae type. It is appar- 

 ent therefore that on grains and plant products, the high ratio V. P. 

 positive, methyl-red negative group predominates. 



Houston, in 1897 and 1898. examined a number of samples of orchard, 

 garden pasture, and virgin soil, including polluted and unpolluted areas, 

 concludes that the true colon bacilli are very rarely or never found in virgin 

 soils, while they are present in large numbers in other areas, especially 

 those which have been contaminated from animal sources. 



Konrich, in a study of 547 specimens of soils in Germany, detected 

 the colon group in 65 percent of 0.1 to 0.5 gram samples. He notes that 



TABL,E XXXVIII. SOURCE, TREATMENT, ETC., OF 42 SAMPLES OF SOIL 



EXAMINED IN 1915. 



I 



II 



III 



IV 



VI 

 VII 



VIII 

 IX 



X 



XI 



XII 



XIII 



XIV 



Corn field... 

 Corn field... 

 Corn field... 

 Corn field... 

 Corn field... 

 Corn field... 

 Expt. plot 

 Expt. plot 

 Expt. plot 

 Expt. plot 



Manured, corn grown for three years 



Manured, corn grown for three years 



Manured, corn grown for three years 



Manured, corn grown for three years 



Manured, corn grown for three years 



Manured, corn grown for three years 



tons manure annually 



114 

 113 

 112 

 111 



Expt. plot 110 



1914 (None 



2 tons manure annually 1914 



1 ton manure annually 1914 



tons of clover chopped and ploughed 

 under annually | 



2 tons of clover chopped and ploughed 



1914 



None 

 None 



None 



under annually 



Expt. plot 109 1 ton of clover as in IV 

 Expt. plot 108 !2 tons of oat straw chopped and ploughed 



under annually 



Expt. plot 107 No treatment, check plot | 



Expt. plot 106 2 tons of timothy chopped and ploughed 



under annually 



Expt. plot 105 jl ton timothy as in IX | 



Expt. plot 104 18 tons of clover once in four years j 



Expt. plot 103 8 tons of manure once in four years 



Expt. plot 102 128 tons peat annually , 



Expt. plot 101 [Timothy grown each year and stubble 

 ' plowed under 



1914 |None 

 1914 JNone 



|None 

 1914 |None 



I 



1914 |None 

 1914 |None 

 1913 |None 



1913 INone 



1914 (None 



1914 (Timothy 



18 

 





 1 





 1 



26 

 24 

 14 



18 



