218 



INFECTIVE DISEASES. 



Involution forms are freely developed in old cultures, and in 

 cultures made in unsuitable media. The bacilli possess numerous 



flagella, and their 



power of movement at 



<0fft once distinguishes them 



from anthrax bacilli. 



4? They can be cultivated 



1 in the ordinary media 



o OM P i n the absence of oxy- 



^ gen, but more readily 



with the addition of 

 grape-sugar or glyce- 

 rine. Radiating fila- 

 f 9" ments grow out from 

 ^ the more or less spher- 

 ical colonies directly 



/ o -* 



V 



>'* 



FIG. 101. BACILLI OF QUAKTER-EVIL x 1000. From 

 an agar culture (FRANKEL and PFEIFFER). 



liquefaction commences. In the depth of 

 nutrient gelatine the growth occurs in two 

 or three days at 20 to 25 0. towards the 

 lower part of the track of the inoculating 

 needle. The gelatine slowly liquefies, and 

 there is considerable formation of gas with 

 the development of a peculiar odour. Spore- 

 formation occurs freely in cultures, but not 

 in the blood of infected animals until after 

 death. 



Guinea-pigs inoculated with a pure- 

 culture, or with spore-bearing threads, die 

 in twenty-four to thirty-six hours. An em- 

 physematous infiltration with sanguineous 

 serum is produced at the seat of inoculation, 

 and the surrounding muscles are of a dark 

 colour. The internal organs are more or less Fl( , m PURE _CULTURE OF 

 congested. The bacilli are found in the BACILLI OF QUARTER-EVIL 



local exudation and in the surrounding IN GRAPE-SUGAR GELA- 

 TINE (FRANKEL 

 PFEIFFER). 



tissue, and some hours 



surrounding 

 after death in 



and 



