DESCRIPTION OF PLATES XVII. AND XVIII. 

 Actinomycosis Bovis. 



PLATE XVII. 



Section of an actinomycotic tongue stained by the method of 

 Gram and with eosin. 



FIG. 1. This illustrates the appearance which is usually seen under a low 

 power, when a section is stained by Gram's method and with eosin. The 

 central portion of a mass of the fungus is either unstained or tinged with 

 eosin, while the marginal portion is stained blue. The reverse is seen, as a 

 rule, in sections from man ; although under a low power the general appear- 

 ance of sections from these two sources is somewhat similar, x 50. 



FIG. 2. a, b, c> d, represent the earliest recognisable forms of the ray fungus 

 in the interior of leucocytes. In e the club-forms can be recognised. In 

 f and g there are small stellate groups of clubs, x 500. 



FIG. 3. A part of the section represented in Fig. 1, under a high power. The 

 marginal line of blue observed under a low power is now recognised as the 

 result of the stain being limited to the peripherally arranged clubs. At 

 () part of a rosette has undergone calcification ; the clubs are granular, 

 and have not retained the stain. At (J) and close to it there are the 

 remains of rosettes in which the process of calcification is almost complete. 

 x 500. 



PLATE XVIII. 



The figures in this plate are taken from sections of a case of 

 so-called " osteosarcoma," in which the growth of the fungus was 

 remarkably luxuriant. The specimens were stained by Plants' 

 method. 



FIG. 1. Different forms of clubs in different specimens : x 1200. 

 O) Very small club-shaped elements. 

 (&) A club with transverse segmentation, 

 (c) A club with lateral daughter clubs. 



(d and <?) Clubs with terminal offshoots resembling teleutospores. 

 (/) A club with developing daughter clubs on the left, and on the 



right a mature secondary club. 

 (# ) A segmental club with lateral offshoots. 

 (1C) Two clubs undergoing calcification. 



FlG. 2. A very remarkable stellate growth comprised of nine wedge-shaped 

 collections of clubs radiating from a mass of finely granular material. 

 x 500. 



FIG. 3. A rosette undergoing central calcification, and consisting in part of 

 extremely elongated clubs resembling paraphyses. Calcareous matter is 

 also being deposited in the club-shaped structures, x 500. 

 FIG. 4. Part of a rosette with continuation of the club-shaped bodies into 

 transversely segmented branching cells apparently representing short 

 hyphae. x 500. 



FIG. 5. A rosette from another section in which similar appearances are 

 observed as in Fig. 4. x 500. 



