444 INFECTIVE DISEASES. 



grains. From some of the tumours, radiating processes of a yellowish 

 colour penetrated into the retro- peritoneal tissue. 



Rotter inoculated calvas, pigs, guinea-pigs, and rabbits. In one 

 case he had a positive result. A piece of actinomycotic growth was 

 introduced into the peritoneal cavity of a rabbit. The animal was 

 killed six months afterwards, and the piece of tissue which had been 

 introduced was found to be encapsuled, arid around it were twenty 

 tumours, from the size of a pin's head to that of a hazel-nut, 

 containing the ray- fungus. 



The author also succeeded in transmitting the disease to a rabbit. 

 A small quantity of human pus, containing the yellow grains, was 

 diffused in broth, and injected with a hypodermic syringe into the 

 abdominal cavity of a rabbit. This rabbit died seventy- nine days 

 afterwards. On examination several nodules were found, about the 

 size of a millet-seed, on the peritoneum of the stomach, in the gastro- 

 splenic omentum, and on the peritoneum of the diaphragm. There 

 was a rounded nodule, about the size of a pea, attached to the 

 stomach. There were adhesions between the intestines, and a 

 tumour about the size of a marble attached to an adhesion of 

 the caecum. One of the small nodules was excised and divided, and 

 the scraping from the interior contained typical rosettes of clubs. 



The successful transmission of actinomycosis from man to bovines 

 suggests intercommunicability, though the negative evidence as to 

 infection of man from bovines supports the view that the disease 

 is derived from some source which is common to both species. 



TRANSMISSION OF ACTINOMYCOSIS FROM CATTLE TO CATTLE. 



Johne was the first to prove that actinomycosis could be trans- 

 mitted from cattle to cattle, and his results were confirmed and 

 extended by Ponfick. 



A calf was inoculated subcutaneously in the neck and cheek, 

 in the gum, and the abdominal cavity. The animal died in forty 

 days after inoculation with development of actinomycosis. 



A calf was inoculated in the cheek and abdominal cavity. Death 

 occurred 114 days after inoculation. In the peritoneal cavity 

 numerous tumours had formed, and the yellowish grains were visible 

 to the naked eye in sections of the new growth. 



A cow in calf was inoculated in the left posterior quarter of the 

 udder ; phlegmonous mastitis followed, and subsided leaving a small 

 induration, which then increased until the inoculated part of the 

 udder was, in three months, nearly double the normal size, from a 



