ACTINOMYCOSIS. , 445 



deposit resembling a fibroma. The cow was slaughtered 133 days- 

 after inoculation. Typical actinomycosis had been produced. 



A colt three and a half years old, was inoculated in the jaw 

 and in the forehead after trephining, and also in the trachea. The 

 animal died without any result. 



Ponfick also conducted a series of experiments which amply 

 confirmed the results which had been obtained by Johne. 



Feeding Experiments. Repeated experiments, made with masses- 

 of the growth chopped- up, or with isolated grains of the fungus, gave 

 negative results. 



Inoculation Experiments. The growth was inoculated in various 

 regions of the body. Small particles of the growth from quite fresh 

 tumours were introduced into the anterior chamber of the eye in 

 rabbits, with negative results. Rabbits were inoculated in the 

 peritoneal cavity from an animal recently slaughtered, but they 

 died of peritonitis. In dogs also the results were negative. 



Seven calves were operated on. In five the abdomen was opened 

 with antiseptic precautions, and in two cases the growth was 

 introduced by injection. In the latter cases the pieces of tumour 

 were suspended in salt solution, but the animals died from peritonitis 

 a few days after the injection. The same result occurred in two out 

 of the five cases in which the abdomen was opened. The three 

 remaining cases gave the following results : 



1. Pieces of tissue, about 1*5 cm. long, were taken from the 

 lower jaw of a recently killed ox. Twelve of these pieces were 

 introduced into the peritoneal cavity; death occurred after 266 

 days, from exhaustion and recent lobular pneumonia. At the post- 

 mortem examination several patches of peritonitis were found, with 

 encystment of the remains of fragments of the inoculated tissue, 

 but there was an independent development of several nodules in the 

 neighbourhood of the stomach and urinary bladder. Examination 

 of these new formations showed, even to the naked eye, that they 

 contained yellowish grains, which, on further examination, proved 

 to be the fungi. 



2. Ten pieces of tumour from the jaw of a cow were introduced 

 as before ; the calf died suddenly sixty days afterwards, during the 

 injection of fresh pieces of actinomycosis into the jugular vein. At 

 the post mortem it was found that various adhesions had occurred, 

 as the result of peritonitis ; and in the false membranes there were 

 sixty-three nodules, varying in size. Microscopical examination 

 showed that all these nodules consisted of typical actinomycotic new 

 formation. 



