GLANDERS. 



4,53 



side of the track of the needle; on glycerine-agar with milk a 

 whitish layer develops, which gradually changes in colour from 

 amber yellow to a reddish-brown. On blood serum the growth is 

 transparent and yellowish ; on potato it is much more characteristic. 

 After two or three days at the temperature of the blood, a film 

 develops in the vicinity of the inoculated area, which is honey-like, 

 transparent and yellow ; the transparency disappears, and in a 

 week the cultures have become reddish-brown. (Plate II., Fig. 6.) 



The disease has been communicated to man by accidental 

 inoculation with a hypodermic syringe which had been used for 

 inoculating cultures. Horses, asses, cats, goats, field mice, and 



FIG. 185. SECTION OF A BRANCH OF THE PULMONARY ARTERY SHOWING 

 GLANDERS BACILLI PENETRATING THE WALL (HAMILTON). 



guinea-pigs, can all be infected with pure cultures ; rabbits, sheep, 

 and dogs are slightly susceptible; cattle, swine, and white mice 

 have an immunity. In the guinea-pig a swelling occurs at the seat 

 of inoculation, followed by the formation of a prominent tumour, 

 developing into an abscess. The skin becomes involved, and an ulcer 

 with indurated margin results. The lymphatic glands also become 

 implicated, and general( infection follows, extending frequently to 

 the testicles or ovaries, and death results after several weeks. In 

 rabbits there is generally only a local abscess induced, which termi- 

 nates in a quickly-healing sore. Mice die in three or four days 

 from general infection ; glanders nodules are found in the liver and 



