APPENDIX IV. 



APPARATUS, MATERIAL, AND REAGENTS EMPLOYED 

 IN A BACTERIOLOGICAL LABORATORY. 



(A) HlSTOLOGICAL APPARATUS. 



Microscope. For the investigation of micro-organisms a good 

 microscope with oil-immersion system and a condenser, such as 

 Abbe's, is essential. Such instruments are supplied by Zeiss, Leitz, 

 Reichert & Hartnack in Germany, and Powell & Lealand, Swift 

 & Baker in England. Zeiss supplies a micrometer eyepiece, with 

 -directions for use. Some such arrangement is essential for the 

 measurement of bacteria. Other accessories to the microscope are : 



A large bell-glass, for covering the microscope when not in use. 



About a foot square of blackened plate-glass. 



A white porcelain slab of the same size. 



Glass bottles, with ground -glass stoppers, for alcoholic solutions of 

 aniline dyes, etc. 



Glass bottles, with funnels, for aqueous solutions of the dyes, and 

 -others provided with pipettes. 



A small rod-stoppered bottle of cedar oil. This is recommended by 

 Zeiss in preference to other oils for his immersion lenses. 



Set of small glass dishes or capsules and watch-glasses, for section- 

 staining, etc. 



Stock of best glass slides, in packets of fifty. 



Several boxes of round and square thin cover-glasses, in various sizes, 

 of the best quality. 



Needle-holders, with a couple of platinum needles, and a packet of 

 ordinary sewing-needles. 



Glass rods drawn out to a fine point ; useful for manipulating sections 

 when acids are employed. 



Platinum or plated copper section-lifters. 



One pair of small brass or spring-steel platinum-pointed forceps, for 

 holding cover-glasses. 



One pair of brass tongg. 



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