APPARATUS, MATERIAL, AND REAGENTS. 615 



preferred by many workers. Smaller accessories, which should be 

 within reach, are 



A small can of sewing-machine oil. 



A soft rag and chamois leather, for wiping the knives immediately 

 after use. 



Stone and leather, for setting and sharpening the same. 

 Two or three camel's-hair brushes. 



A freezing microtome is very useful : such as Swift's, which is 

 used by the author; and the method of embedding in celloidin is 

 combined with the ordinary process of freezing. 



(B) REAGENTS AND MATERIAL EMPLOYED IN THE PROCESSES or 

 HARDENING, DECALCIFYING, EMBEDDING, FIXING AND CUTTING 

 OF TISSUES. 



Alcohol, absolute. 

 Bergamot oil. 



Celloidin. 



Dissolved in equal parts of ether and alcohol. 



Cork, or stock of ready-cut corks. 



Ebner's solution. A mixture in the following proportions : 



Hydrochloric acid .... '5 



Alcohol ,100 



Distilled water 20 



Chloride of sodium ... 5 



Formalin. 



Gelatine. 



Melted in a small porcelain capsule, and set aside ready to be 

 re-melted when required for use. 



Glycerine gelatine (Klebs). 



Best well- washed gelatine . . . .10 



Add distilled water, allow gelatine to swell up, pour 



off excess of water, melt gelatine with gentle heat, 



add 

 Glycerine ..... .10 



Lastly, a few drops of phenol for preservation. 



Gum. 



