618 APPENDICES. 



17. Gibbes' solution, for double staining. 

 Take of 



Rosaniline hydrochlorate ..... 2 



Methylene-blue ...... 1 



Triturate in a glass mortar. 



Dissolve aniline oil . . . . . 3 



In rectified spirit ... . . . .15 



and add slowly to the above. 



Lastly, slowly add distilled water . . .15 

 Keep in stoppered bottle. 



18. Glycerine, pure. 



19. Hsematoxylin solution. 



Hsematoxylin . . . . . . . 2 



Alcohol 100 



Distilled water . . . . . .100 



Glycerine ....... 100 



Alum ........ 2 



20. Iodine solution. 



Iodine, pure ....... 1 



Iodide of potassium ...... 2 



Distilled water 50 



21. Iodine solution (Gram). 



Iodine ..... 1 



Iodide of potassium . . . . . 2 

 Distilled water 300 



22. Lithium-carmine solution (Orth). 



Saturated solution of carbonate of lithium . 100 

 Carmine ...... . 2'5 



23. Magenta solution (Gibbes). 



Magenta ...... 2 



Aniline oil ... . 3 



Alcohol (sp. gr. -830) 20 



Distilled water .... 20 



24. Methylene-blue. 



(a) Concentrated alcoholic solution. 



{b) Aqueous solution. 



Methylene-blue .... 2 



Alcohol 15 



Water . . 85 



