APPARATUS, MATERIAL, AND REAGENTS. 619 



(c) Koch's solution. 



Concentrated alcoholic solution of methylene-blue 1 

 Ten per cent, potash solution .... 

 Distilled water 200 



(d) Loffler's solution. 



Concentrated alcoholic solution of methylene-blue 30 

 Solution of potash, 1 to 10,000 . . . 100 



25. Methyl-violet. 



(a) Concentrated alcoholic solution. 



(b) Aqueous solution. 



Methyl- violet . . . . . . . 2 -2 5 



Distilled water ... . . .100 



(c) Koch's solution. 



Aniline water . . . . . . .100 



Alcoholic solution of methyl-violet . .11 



Absolute alcohol . . . . . .10 



26. Neelsen's solution. 



Dissolve f uchsine ..... 



In alcohol 10 



Add a 5 per cent, watery solution of carbolic acid 100 



27. Nitric acid, pure. 



28. Orseille (Wedl). 



Dissolve pure ammonia-free orseille in 



Absolute alcohol ... .20 



Acetic acid .... 5 



Distilled water . 40 



until a dark red liquid results. Filter. 



29. Picric acid. 



(a) Concentrated alcoholic solution. 



(b) Saturated aqueous solution. 



30. Picro-carmine (Ranvier). 



Carmine . 



Distilled water . .10 



Solution of ammonia 3 



Triturate ; add cold saturated solution of picric 

 acid .... 200 



