IMMUNIZATION BY MEANS OF THE BACTERIOPHAGE 267 



August 8. The intestinal bacteriophage was active as follows: B. coli 

 -f ++, Shiga +, Flexner 0, Hiss +. 



August 9. The patient was discharged from the hospital. 



Andre" B. . . . (ten years). A case of bacillary dysentery of moderate 

 severity. During the period from August 25 to 29 inclusive there were 9 

 to 11 bloody stools a day. 



August 28. Stool examination showed: B. dysenteriae Shiga present. 



Intestinal bacteriophage active as follows: B. coli +, Shiga 0, Flexner 

 0, Hiss 0. 



August 29. At 4 p.m. the patient ingested 2 cc. of an anti-Shiga bac- 

 teriophage culture. The culture had been lysed for two months. 



August 30. There was one bloody stool in the morning and during 

 the afternoon and the night there were 5 stools, none of which showed 

 any blood. 



August 31. There were 3 fluid, but not bloody, stools. 



Examination showed: Shiga bacilli not present. 



The intestinal bacteriophage active as follows: B. coli + + ++, Flexner 

 ++, Hiss +++, Shiga + + + + . 



September 1. There was one fluid stool, without blood. 



September 2. There was one fluid stool, without blood. 



September 3. There was one formed stool. Examination of the intes- 

 tinal bacteriophage showed: B. coli +++, Shiga ++++, Flexner + ++, 

 Hiss +. 



September 8. Reactions with the intestinal bacteriophage were: 

 B. coli ++, Shiga 0, Flexner 0, Hiss +. 



September 9. The patient was discharged from the hospital. 



Robert D. . . . (twelve years). This patient had a very severe dysen- 

 tery, with vomiting, cold sweats, chilling of the extremities, and involun- 

 tary and uncountable stools. 



September 8. The stools could not be counted. They were fetid, 

 purulent, and streaked with blood. Examination showed: B. dysenteriae. 

 Shiga present; about 1 out of every 10 colonies on the plates was the dysen- 

 tery bacillus. 



The intestinal bacteriophage showed no virulence for B. coli, or for the 

 Shiga, Flexner or Hiss organisms. 



September 9. Two cubic centimeters of an anti-Shiga bacteriophage 

 culture were ingested at 11 o'clock. This culture had been lysed for three 

 and one-half months. During the afternoon and the night the stools 

 became less numerous but continued bloody. 



September 10. There were 6 fluid stools, without blood. Examination 

 showed: B. dysenteriae Shiga, not present. 



Intestinal bacteriophage active as follows: B. coli + +++, Shiga 

 + + ++, Flexner ++++, Hiss + + + . 



September 11. There were 2 normal, formed stools. 



September 20. The patient was discharged from the hospital. 



