To Stain with Logwood. 23 



LOGWOOD STAIN. 

 1. Take of 



Extr. Hsematoxyl, grms. 6 

 Alumen, grms. 18. 



Mix thoroughly, while mixing add 28 c.c. of distilled 

 water. Filter and add to the filtrate 3 j of spirit of 

 wine. Keep in a stoppered bottle a week before using. 

 What remains on the filter can be mixed with 14 c.c. of 

 distilled water, and left soaking in it for an hour or so, 

 then filter and add to the filtrate 3 BS of spirit of wine. 

 This second solution is as strong as the first. 



To STAIN WITH LOGWOOD. 



Make a cone with a small round filter paper, and 

 pour some of the staining fluid into it, let from seven to 

 ten drops fall into a watch glass and dilute with dis- 

 tilled water. Let the sections remain in the solution for 

 about a quarter of an hour, the time will depend on the 

 tissue and the manner in which it has been hardened. 

 Some tissues take in the stain very rapidly, others 

 slowly. Take out a section from time to time, and place 

 it in a watch glass of ordinary filtered water to see if it 

 is stained deeply enough. 



When the sections appear to have stained thoroughly 

 remove them to a watch glass of ordinary water, and 

 wash them to remove the excess of colouring matter. 

 In staining with logwood it is necessary to be careful 

 that too many sections are not placed in the solution 

 at once, as they will lie thickly one on the other, and 

 the staining will not be uniform ; it is also necessary to 



