26 On Staining. 



and not easily obtained. It is soluble in spirit with 

 the addition of slight heat. 



Place some of the powder in a test tube and add 

 some rectified spirit, warm gently over a spirit lamp. 

 When dissolved, filter and keep in a stoppered bottle. 

 It stains readily and brings out some tissues such as 

 muscle and mucous glands well. 



It does very well in double staining with aniline 

 blues. 



5. Anthra-Purpurine. A brown powder which is 

 often sold for purpurine. It is isomeric with it, but 

 is found as a secondary product in the preparation of 

 alizarin from anthracene. 



It is very slightly soluble in water and may be re- 

 moved entirely from aqueous solutions by means of 

 ether. 



A solution may be made by first dissolving it in 

 spirit and then adding a warm solution of alum in 

 water, the color will then become reddish. It stains 

 muscle and epithelium but not connective tissue. 



It does not show up minute structures as well as 

 logwood. 



It does not combine with picro- carmine in double 

 staining, but the difference in the two colors is so slight 

 as to make this of little value. 



6. Eosin. Is not an anilin color but is a potash salt 

 of resorcin. Kesorcin is obtained by the action of 

 melting potash on galbanum. Eosin is largely soluble 

 in water and has a beautiful garnet red color. In using 

 it a strong solution is required and the sections must 

 be well washed in water after staining. It is a very 



