On Staining. 29 



granules, and placing them in a large test-tube, and 

 adding distilled water : the solution ought to be strong, 

 nearly saturated. When all the granules have dis- 

 solved, filter the solution, and keep it in a stoppered 

 bottle. 



Sections that are to be passed through spirit require 

 to be rather deeply stained, as a good deal of the colour- 

 ing matter will come out in the spirit, and it is better 

 to remove them at once from the water to absolute 

 alcohol, as the colour is not affected so much by it. 



9. Pure soluble Anilin Blue. This is a very good blue 

 colour, soluble in water : it is more brilliant than the 

 former, and more expensive ; the China blue is, in fact, 

 prepared from it. A saturated solution must be made 

 and great care used in staining with it, as its action is 

 very rapid. Like all anilin colours, its brilliancy is 

 impaired by a long immersion in spirit. 



This colour has not been much used as yet, but it 

 will probably become a favourite when it is better 

 known. To use either of these solutions, a few drops 

 are placed in a watch-glass, and diluted with water, 

 one or two drops are quite sufficient, the sections are 

 then washed well in water. 



10. Serge Blue. This is another shade of blue ; a 

 good colour, and stains well, but will not stand immer- 

 sion in spirit. 



11. Tyrian Blue. A much less brilliant colour than 

 any of the others, more inclined to purple : it does not 

 stand spirit, and cannot be much used until some 

 method is found for fixing it. 



12. Safranine. Is a good deep colour, and stains 



