32 On Staining. 



the section must be very deeply stained to get enough 

 left in it to make a satisfactory specimen. 



There are several other colours soluble in spirit which 

 are much easier to use and consequently this has not 

 been much tried, but it would well repay the trouble 

 if some means were found to make it more permanent. 

 A saturated solution must be used, and it is better not 

 to wash the section too much in spirit, before putting 

 it in the aqueous solution in double staining. 



18. Rosein. This is the best of all the anilin colours 

 soluble in spirit and must not be confounded with ros- 

 anilin. It is easily soluble in spirit, the ordinary 

 methylated spirit will do, its action is rapid, and it is 

 better to use a strong solution and immerse the section 

 for a very short time. 



To make the solution, place some of the granules in 

 a large test tube and nearly fill with spirit, cork and 

 shake it and let it stand, when all the granules are 

 dissolved, add some more until the solution appears 

 to be saturated, then filter and keep in a stoppered 

 bottle. Place some in a watch glass and dilute with 

 about an equal quantity of spirit ; this will do for all 

 ordinary purposes. 



19. Anilin Eed. This is another shade of red and 

 does not differ much from rosein in colour while it has 

 not its universal applicability, and does not fix so well. 

 It has not been much used, and experiments might 

 bring out some valuable points in it as a dye for special 

 subjects. It is prepared and used in the same manner 

 as rosein. 



20. Anilin Violet. This colour comes next to rosein 



