36 Picro- carmine and Anilin. 



and time for experimenting are more required, than in 

 double staining. 



The student must not be discouraged by many 

 failures as there is always some new fact to be learnt 

 and noted, and in the application of these to future 

 experiments, some brilliant results are sure to be ob- 

 tained. 



PlCKO-CAKMINE AND LOGWOOD. 



This combination has been used for a long time, and 

 gives very satisfactory results. The student should 

 begin with sections of scalp, skin, or tongue, and the 

 result, if the process be carefully carried out, will be 

 found very satisfactory. 



The sections must be first stained in picro- carmine 

 and then in logwood. Make a dilute solution of picro- 

 carmine in distilled water, about 10 drops to the watch 

 glass, and let the sections remain in it for from twenty 

 minutes to half an hour, then wash in water and place 

 in distilled water acidulated with 1 or 2 drops of acetic 

 or picric acid. Let them remain in this for about an 

 hour. Eemove the sections from the acidulated water 

 and place them in dilute logwood stain ; this should not 

 be too strong, from 5 to 7 drops to the watch glass of 

 distilled water; do not let them stain too deeply. 

 When sufficiently coloured, which will be shown by 

 their becoming a faint lilac colour, they must be 

 washed to remove the excess of logwood, and mounted 

 in the usual manner. 



This double stain is very effective when used with 

 fresh tissues, such as serous membranes ; it brings out 



