Treble Staining. 39 



in acidulated water for an hour, and afterwards stain- 

 ing them with various solutions of anilin colours. 



Safranine, after picro-carmine, gives a good double 

 stain, as the picro-carmine colours all the connective 

 tissue and nuclei, while the safranine stains muscle, 

 epithelium, &c. ; but the two colours are not sufficiently 

 different to give as good a result as logwood and picro- 

 carmine, although they will be found useful where great 

 transparency is desired. 



Picro-carmine and iodine green give a very beautiful 

 effect when it is wanted to isolate gland tissue ; such 

 as Peyer's patches, or the glands in the tongue, oeso- 

 phagus, or solitary glands in the large intestine. The 

 picro-carmine staining everything but the glands, which 

 remain a bright green. 



Eosin and anilin blue give good results, but require 

 to be used cautiously, as if the staining is too deep the 

 section becomes opaque. To get the best effect, the 

 section should be very thin, and must be well washed 

 after staining with eosin, and then just immersed for a 

 few seconds in the anilin blue. 



A great many other combinations will suggest them- 

 selves to the student, and he will be amply rewarded by 

 experimenting further with the various staining agents 



mentioned. 



TREBLE STAINING. 



The combination which has given the best effect so 

 far in treble staining is : picro-carmine, rosein, and 

 iodine green. Stain the sections well according to the 

 process already described for picro-carmine, and soak 

 them in acidulated water. Then take a few drops of 



