42 Chloride of Gold and Anilines. 



which colour predominates. In the serous glands, how- 

 ever, quite another aspect is presented; there is no 

 combination as in the mucous glands, but the proto- 

 plasm of the cells is stained more or less deeply with 

 rosein, while the nuclei have taken on the green ; the 

 colour differs, however, from that of the surface epithe- 

 lium, and appears to have taken on picro-carmine to 

 some extent, which, with the rosein, gives a dull red 

 colour. 



In many places will also be seen small masses of 

 adenoid tissue which have stained a bright green 

 throughout. 



Altogether this makes one of the most brilliant speci- 

 mens in the whole range of histology, and although the 

 process is rather troublesome, and requires a certain 

 amount of practice to determine the time required for 

 each immersion, it amply repays when once properly 

 done. 



Take only a few sections at a time, and do not hurry 

 over the different processes, and after a few trials the 

 exact time of immersion will be hit on, and should be 

 recorded. 



CHLORIDE OF GOLD AND ANILINES. 



Some very striking results may be obtained by first 

 staining fresh tissues, especially growing bone, in chlo- 

 ride of gold solution (page 7), and then decalcifying 

 and hardening in spirit. After the material has har- 

 dened sufficiently, sections may be made and stained 

 with two colours. It is not quite clear what action the 

 gold chloride has on those parts it does not stain, but 



