On Mounting. 53 



After the cover glass has been removed the section 

 must be floated off into chloroform until all the balsam 

 or Dammar has been dissolved out of it, and then placed 

 in alcohol for a short time ; it may then be re- stained if 

 necessary and mounted again. 



MOUNTING LAKGE SECTIONS. 



In manipulating large sections it is rather difficult to 

 pass them through the different processes without in- 

 jury. This may generally be done with care, and they 

 may even be double stained. 



There are some tissues, however, so fragile that they 

 cannot be lifted on the needle without tearing, and 

 these must be left in one vessel and the different pro- 

 cesses applied to them there. This is not a very satis- 

 factory method, as the staining cannot be so well done 

 unless a large quantity of fluid is used, and every section 

 carefully separated from its neighbour, they are also apt 

 to be injured in pouring off the different fluids. This is 

 only required in exceptional cases. It is when the mount- 

 ing from the oil of cloves comes, that the difficulty is 

 experienced ; as, even if a lifter is specially made large 

 enough to take up a whole section, the adhesion of the 

 section to such a large surface is so great, that it is im- 

 possible to get it off without tearing, if the section is as 

 thin as it ought to be. It may, however, be done by 

 using the cover glass as a lifter in the following man- 

 ner. 



Take as an example a longitudinal section of kidney 

 of large dog or man, having been safely stained, it lies 

 in the oil of cloves ready to be transferred to the slide. 



