CHAPTEE VII. 

 METHOD OF OBTAINING ANIMAL TISSUES FOE EXAMINATION. 



THE animals required will be a cat, rabbit, and guinea 

 pig ; and for some special tissues, a frog, salamander, 

 and newt. 



To kill the first three animals, place them in .a 

 box with a tight- fitting lid, having previously intro- 

 duced a sponge saturated with chloroform. In this 

 confined space the chloroform will render the animals 

 insensible in a very short time, and then kill them 

 without inflicting the slightest pain. 



The other three animals may be chloroformed under 

 a small bell glass on a plate. 



Having killed either of the first animals, make an in- 

 cision through the skin, from the chin to the anus, and 

 reflect it on each side, open the abdomen and remove 

 some of the mesentery and omen turn for preparation by 

 the silver process ; open the thorax and make an in- 

 cision into the heart and drain off the blood before it 

 coagulates. Before opening the heart a small piece of 

 the centrum tendineum of the diaphragm may be care- 

 fully cut out to be prepared by the silver process. Then 

 open up the thorax and abdomen thoroughly. Take out 

 the lungs with a small portion of the trachea attached. 

 Kemove the heart, open up the ventricles. Eemove the 

 liver and cut it into small pieces. Take out the sto- 

 mach with a piece of oesophagus and duodenum at- 

 tached, open it longitudinally and wash it in dilute 



