10 Cartilage. 



lacunae ; but in hardened specimens they have all 

 shrunk, more or less, leaving a space. 



Specimens of cartilage should also be hardened in 

 chromic acid mixture, and thin sections stained with 

 logwood. 



FlBKO- C AKTILAGE . 



Make a longitudinal section of mouse's tail, and 

 notice the intervertebral cartilage and its gradual tran- 

 sition into hyaline cartilage on the bone. 



The fibres will be seen in various aspects as they 

 cross one another, and several sections should be ex- 

 amined; the ceUs will be seen lying between the fibres. 

 Make a section of the intervertebral disc of sheep or ox, 

 hardened in chromic acid mixture, and stain with log- 

 wood. Mount some sections whole and tease out others 

 on the slide. Cover and examine. It will be difficult 

 to make out the fibres in some sections. 



ELASTIC CARTILAGE. 



This can be well shown in the lobe of the ear or in 

 the epiglottis. Procure the epiglottis of a sheep and 

 harden it in the chromic acid mixture, cut sections and 

 stain them with logwood. 



The ear of a child prepared in the same manner, sec- 

 tions cut and stained with logwood. 



The ear lobe of a pig also prepared in chromic acid 

 mixture and stained with logwood. Sections of these 

 must be thin and they must not be hardened too much 

 or it will be difficult to cut them. 



The pig's ear when well prepared makes a very use- 

 ful specimen, as it shows a great many tissues. 



