72 Muscular Tissue. 



according to size, to get rid of the lime salts, and then 

 preserved in spirit. 



Bone may be macerated in a saturated solution of 

 picric acid, but it does not act so well as the above. 

 It must be kept saturated by the addition of fresh crys- 

 tals. 



Make longitudinal sections through the head of a 

 long bone such as the femur of a small kitten, double 

 stain with picro- carmine and logwood and mount in 

 Canada balsam. Take a portion of the lower jaw of a 

 very young kitten near the condyle and decalcify it. 

 When prepared cut transverse sections and double 

 stain them with picro- carmine and logwood and mount 

 in Canada balsam. Sections also through the carpus 

 and tarsus of a foetal child or kitten may be made and 

 stained in the same manner. 



The easiest specimens of growing bone to be got are 

 from kittens just born, they should be decalcified in 

 chromic acid as mentioned before. 



MUSCULAE TISSUE. 

 NON-STKIPED, STRIPED, HEART MUSCLE. 



1. Non-striped Muscle. Make a preparation of the 

 mesentery of newt or salamander in chromate of 

 ammonia, page 13, and mount in glycerine. This 

 specimen shows better when double stained with picro- 

 carmine and logwood, page 36. 



Examine first with a low power and see the distri- 

 bution of the muscle fibres through the mesentery, and 

 then with a high power to see the structure of the in- 



