Nervous Structures. 77 



To SHEW THE LARGE MULTIPOLAB NERVE CELLS IN THE 

 SPINAL CORD. 



Cut a piece of spinal cord through the middle longitu- 

 dinally, take one side and holding it in the hand cut 

 out with a sharp razor as thin a slice as possible of the 

 anterior column, stain this deeply in picro- carmine. 

 Take a small portion and place it in a drop of gly- 

 cerine on a slide and tease out very carefully with two 

 needles, this must be done gently and continued for 

 some time until the whole piece is reduced to very 

 minute portions, hardly discernible except by their 

 colour ; cover and seal up. 



If this process has been carefully done, the large cells 

 will be seen stained with picro-carmine and completely 

 isolated with processes of different lengths, according to 

 the care which has been exercised in teasing. 



Several sections of the anterior column should be 

 made and after staining they should be placed on a 

 slide and examined with a low power ; those which con- 

 tain a large number of multipolar cells, will be readily 

 seen. 



BRAIN. 



Small brains, such as rabbit's, harden very well whole 

 by the spirit process, page 13, they must then be placed 

 in absolute alcohol, before doing this the different parts 

 should be separated, viz : frontal lobes, cerebellum, 

 pons, &c. 



After remaining in the alcohol a few days, sections 

 may be cut of any of the parts to be examined and 



