102 Hydatids. 



cut a moderately thick slice, say about a quarter of an 

 inch thick, and harden it in chromic acid mixture, than 

 to harden the whole mass in spirit, as it will be found 

 a very difficult matter to cut large sections of spirit 

 hardened material ; they become so hard that they 

 make the knife jump, and the section is consequently 

 uneven. 



AMYLOID DEGENEKATION 



Is best hardened in chromic acid mixture, and in 

 kidney especially is very well shown by this method. 

 To show the amyloid substance well it must be stained 

 a different colour to the surrounding tissue, and this 

 may be done by several of the double staining pro- 

 cesses ; as the anilin dyes seem to have a special af- 

 finity for amyloid degeneration, the following should 

 be tried : 



Carmine and indigo carmine (page 87). 

 Eosein and iodine green. 

 Eosin and anilin blue. 



and many other combinations. 



HYDATIDS. 



To make a preparation of hydatid cysts, take a por- 

 tion of the wall of a large cyst and scrape off some of 

 the gelatinous matter adhering to it. Place a little of 

 this on a slide and tease it gently in a drop of glycerine, 

 cover and examine. If there are any small cysts show- 

 ing booklets, &c., well, seal it up with HolhV glue. 



