BLOOD ANALYSIS 



Transferral of the Diluted Blood to the Chamber, The counting chamber which 

 has been cleaned with distilled water and alcohol-ether and then wiped dry with 

 a soft cloth as free from lint as possible is placed upon a black surface and carefully 

 brushed with a camel's hair brush. The cover-glass is now placed over the chamber 

 by sliding it over the two glass plates with both thumbs while the index-fingers are 

 pressing it down. By means of the clamps it is held in place firmly so that New- 

 ton's rings (if possible of the first order: brown and black) may be seen over the 

 entire area of the plates. The chamber is placed upon the stage of the microscope 

 and is brought into a horizontal position. 



Before transferring the diluted blood to the chamber the flask must be shaken 

 for two minutes as described before. The liquid shows a cloudy appearance and 

 must be allowed to stand until the turbidity has become uniform. 



One of the plain pipettes described above is now inserted into the diluted blood 



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T 



t 

 FIG. 1 01. SCHEMA. 



13 



13 



13 



while slight pressure is being exerted on the rubber cap. The pressure is released 

 slowly and the liquid rises into the pipette. The point of the pipette is now im- 

 mediately placed upon one of the projecting ends of the floor plate and very slight 

 pressure is exerted on the rubber cap until the liquid coming from the pipette just 

 reaches the cover-glass when the pressure is released. An instantaneous filling 

 of the capillary space results. The pipette should be emptied immediately, rinsed 

 with distilled water and placed in an upright position in a beaker of water. The 

 other portion of the counting chamber is now filled in the same way with a second 

 pipette and about one minute is allowed for the settling of the corpuscles. During 

 this time the pipettes may be washed with distilled water and ether-alcohol and 

 dried by suction. Occasionally, the pipettes should be cleaned with a horse hair 

 and with concentrated H 2 SO 4 containing a little K 2 Cr 2 O 7 . 



To see whether the distribution. of the corpuscles has been uniform the chamber 

 is illuminated with a wide-open diaphragm and viewed at an angle. If the opacity 



