338 



PHYSIOLOGICAL CHEMISTRY 



BONE ASH. 



Add dilute nitric acid, stir thoroughly and after the major portion of the ash has been 

 brought into solution add a little distilled water and filter. 



Residue I. 



(discard) 



Fatrate I. 



Add ammonium hydroxide to 

 alkaline reaction and filter. 



I 



Residue n. 



Treat on paper with acetic acid. 



Residue HI. 



Treat on paper with hydro- 

 chloric acid. 



Filtrate IV. 

 Test for: 



1. Iron. 



2. Phosphates. 



FUtrate HI. 



Test for: 



1. Phosphates. 



2. Calcium. 



3. Magnesium 



V. ADIPOSE TISSUE 



Fatrate H. 



Test for: 



1. Chlorides. 



2. Sulphates. 



3. Phosphates. 



4. Calcium. 



Adipose tissue consists almost entirely of a mixture of fats, 

 discussion and experiments see chapter on Fats, page 176. 



For 



