566 



PHYSIOLOGICAL CHEMISTRY 



of Analysis, see Chapter XXVI). Total solids, titratable acidity, hydrogen ion 

 concentration, total nitrogen, amino-acid nitrogen, ammonia, urea, uric acid, 

 creatinine, total sulphur, ethereal sulphates, inorganic sulphates, neutral sulphur 

 (by difference) total phosphates and sodium chloride. 



Calculate the nitrogen and sulphur "partitions," i.e., the percentage of the 

 total nitrogen and sulphur which occur in the different forms and tabulate the data 

 from the complete analysis. Compare your results with those listed in the table 

 on pages 371 and 578. 



3. Hyperglycemia Produced by Carbohydrate Ingestion. The 



average glucose content of normal blood is somewhat less than o.i per 

 cent. This is increased (hyperglycemia) on the ingestion of carbo- 

 hydrate food. The increase is noted more quickly after the ingestion of 



0.18 



Hours 



FIG. 169. BLOOD SUGAR AS INFLUENCED BY DIET. 

 A = glucose; B = starch; 

 C = starch and fat; D fat. 



monosaccharides than after the ingestion of the more complex carbo- 

 hydrates. After the ingestion of 100 grams of glucose or starch an 

 increase in the sugar of the blood sometimes occurs in five minutes. 1 

 (See Fig. 169.) 



(a) Influence of Glucose. In the morning before breakfast, or three to five 

 hours after breakfast, determine the normal sugar content of your blood by means 

 of some accurate micromethod. (See Chapter XVI.) Ingest 100 grams of 

 glucose dissolved in 250 c.c. of water, and again determine the blood sugar 

 level at intervals, of 5, 15 and 30 minutes and one, two and three hours. (Plot 

 a curve similar to the one shown in Fig. 169. The urine may also be examined 

 for sugar at intervals of one hour after the sugar ingestion. 



Repeat the experiment on another day using 250 grams of glucose and com- 

 pare the results with those obtained after the ingestion of 100 grams. Explain 

 your findings. If desired this experiment may be combined with the ones on 

 41 Alimentary Glycosuria," and "Carbohydrate in Feces," see pages 568 and 591. 



1 Jacobson: Bioch. Zeit,, 56, 471, 1913. 



