PHYSIOLOGICAL CHEMISTRY 



BiaPs Reagent. 1 



Orcinol 1.5 grams. 



Fuming HC1 500.00 grams. 



Ferric chloride (10 per cent) 20-30 drops. 



Biuret Reagent, Gies. 2 This reagent consists of 10 per cent KOH 

 solution to which 25 c.c. of 3 per cent CuSO4 solution per liter has been 

 added. This imparts a slight though distinct blue color to the clear 

 liquid. 



Biuret Paper (Kantor and Gies). 2 Immerse filter paper in Gies' 

 Biuret Reagent (above) then dry and cut into strips. 



Black's Reagent. 3 Made by dissolving 5 grams of ferric chloride 

 and 0.4 gram of ferrous chloride in 100 c.c. of water. 



Blood Serum. This may easily be obtained in quantity by the 

 procedure described under Hemagglutination in the chapter on Blood. 



Boas' Reagent 4 Dissolve 5 grams of resorcinol and 3 grams of 

 sucrose in 100 c.c. of 50 per cent alcohol. 



Bonnano's Reagent Dissolve 2 grams of sodium nitrite in 100 c.c. 

 of concentrated hydrochloric acid. 



Bottu's Reagent. To 3.5 grams of 0-nitrophenylpropiolic acid 

 add 5 c.c. of a freshly prepared 10 per cent solution of sodium hydroxide 

 and make the volume of the solution i liter with distilled water. 



Carmine-Fibrin. 5 Prepared by running fibrin through a meat 

 chopper, washing carefully and placing in % per cent ammoniacal 

 carmine solution (very little excess ammonia should be present) until 

 the maximum coloration of the fibrin (dark red) is obtained. The fibrin 

 is then washed in water and in water acidified with acetic acid. It is 

 preserved under glycerol. 



Chloride Reagents for Blood Analysis. 6 (a) An acid M/2Q.25 

 solution of silver nitrate, i c.c. of which is equivalent to 2 mg. of NaCl. 



AgNO 3 5.812 grams. 



HNO 3 (sp. gr. 1.42) 250 c.c. 



Water to 1000 c.c. 



(b) A solution of M/58.5 potassium iodide, i c.c. of which is equiva- 

 lent to i mg. of NaCl. 



KI 3-o grams. 



Water to 1000 c.c. 



This solution is standardized against the silver solution by adding 5 c.c, 

 of the latter to 5 c.c. of solution (c) and titrating with the iodide solution 



1 Test for pentose, p. 37. 



2 Protein tests, p. 100. 



3 Black's reaction, p. 441. 



4 Test for free acid, p. 154. 



5 Tests on proteases, p. 12. 



6 Method of McLean and Van Slyke, p. 286. 



