608 PHYSIOLOGICAL CHEMISTRY 



Spiegler's Reagent. 1 This reagent has the following composition: 



Tartaric acid 20 grams. 



Mercuric chloride 40 grams. 



Sodium chloride 50 grams. 



Glycerol 100 grams. 



Distilled water 1000 grams. 



Starch Iodide Solution. 2 Mix o.i gram of starch powder with 

 cold water in a mortar and pour the suspended starch granules into 75- 

 100 c.c. of boiling water, stirring continuously. Cool the starch paste, 

 add 20-25 grams of potassium iodide and dilute the mixture to 50 c.c. 

 This solution deteriorates upon standing, and therefore must be freshly 

 prepared as needed. 



Starch Paste. Grind 2 grams of starch powder in a mortar with a 

 small amount of water. Bring 200 c.c. of water to the boiling-point and 

 add the starch mixture from the mortar with continuous stirring. Bring 

 again to the boiling-point and allow it to cool. This makes an approxi- 

 mate i per cent starch paste which is a very satisfactory strength for 

 general use. 



Stokes* Reagent. 3 A solution containing 2 per cent ferrous sulphate 

 and 3 per cent tartaric acid. When needed for use a small amount 

 should be placed in a test-tube and ammonium hydroxide added until 

 the precipitate which forms on the first addition of the hydroxide has 

 entirely dissolved. This produces ammonium ferrotartrate, which is a 

 reducing agent. 



Suspension of Manganese Dioxide. 4 Made by heating a 0.5 per 

 cent solution of potassium permanganate with a little alcohol until it is 

 decolorized. 



Tanret's Reagent. 5 Dissolve 1.35 grams of mercuric chloride in 

 25 c.c. of water, add to this solution 3.32 grams of potassium iodide 

 dissolved in 25 c.c. of water, then make the total solution up to 60 c.c. 

 with distilled water and add 20 c.c. of glacial acetic acid to the mixture. 



Tincture of Iodine. 6 Dissolve 70 grams of iodine and 50 grams of 

 postassium iodide in i liter of 95 per cent alcohol. 



Toison's Solution. 7 This solution has the following formula: 



Methyl violet o . 025 gram. 



Sodium chloride i . o gram. 



Sodium sulphate 8.0 grams. 



Glycerol 30.0 grams. 



Distilled water 160 . o grams. 



1 Spiegler's ring test, pp. 104 and 424. 



2 Fehling's method, p. 523. 



3 Hemoglobin, p. 206. Hemochromogen, p. 299. 



4 Kriiger and Schmidt's method, p. 513. 

 6 Tanret's test, pp. 104 and 425. 



6 Smith's test, pp. 268 and 433. 



7 "Blood counting," p. 305. 



