MORBID ANATOMY 6 1 



Bacteriological notes. At the autopsy an agar tube was inoculated 

 with a platinum loop lightly rubbed over the pleural exudate. On the 

 following day a thin grayish growth with condensation water clouded. 

 Examination of hanging drop and stained coverglass preparations shows 

 only swine-plague germs. 



In coverglass preparations of hepatized lung tissue a large number 

 of germs resembling swine-plague bacteria were seen. 



With a bit of hepatized lung tissue, a rabbit was inoculated subcu- 

 taneously at 2 p. M. The rabbit was dead next morning at S a. m., i. e., 

 in less than i8 hours. In spleen, liver, and blood preparations numer- 

 ous polar-stained swine-plague germs present. An agar culture from 

 heart's blood contained only swine-plague germs. 



From the peritoneal exudate of pig, consisting of cells and fibrin 

 and numerous bacteria of several varieties, two agar plate cultures were 

 made. On Plate A one large colony of spore-bearing bacilli and one 

 small colony of swine-plague germs. Plate B, completely overgrown by 

 the spore-bearing bacillus. 



A bouillon culture from the exudate contains streptococci and 

 swine-plague bacteria. Agar plate cultures were made therefrom and 

 both germs isolated. 



At the same time a large rabbit was inoculated subcutaneously with 

 a bit of the exudate Dead in i8 hours. Stained coverglass prepara- 

 tions of blood from heart, spleen, and liver tissue show polar-stained 

 swine-plague germs. An agar culture from the blood contains onh' 

 swine-plague germs. 



From the spleen, after thoroughly scorching through the exudate, 

 two agar plate cultures and a bouillon culture were made with bits of 

 spleen pulp. The bouillon culture contained only swine-plague germs. 



The swine-plague bacteria were thus obtained from lungs, pleura, 

 spleen, and peritoneal exudate, while hog-cholera bacilli, which were 

 looked for with the greatest care, could not be found." 



^ 50. Differential diagnosis. In sporadic cases, swine 

 plague is to be differentiated from broncho-pneumonia due 

 to other causes than the swine-plague bacterium. Pneumonia 

 of a non-specific nature is often associated with deaths due to 

 dietary or other causes. 



In epizootics or outbreaks it is to be distinguished from 

 hog cholera when there is accompanying catarrhal pneumonia. 

 To make a positive diagno.sis it is usually necessary to depend 

 upon the results of the bacteriological examination. 



Pneumonia resulting from lung worms, (Strongylus para- 

 doxus) can be distinguished by a careful examination of the 

 contents of the bronchioles. 



